Thin layer chromatography and column chromatography

Then you open the tap again so that the coloured mixture is all absorbed into the top of the packing material, so that it might look like this: In general, this shows that the components making up the sample were more polar than non-polar, but each had a different polarity from the other.

The pure fraction of pyrene was combined in a pre-weigh test tube and the pure fraction of p-nitroaniline in another test tube. You can also draw a thin line with pencil.

This can be eliminated by diluting the sample solution. If a standard of the target compound is available, it is good practice to produce a co-spot by spotting the standard onto a spot of the unknown mixture.


Comparison of the unknown with reference standards 1. The mobile phase is the phase that moves in a definite direction. A column containing a fritted disk and a stop cock was assembled with two clamps to stand vertically in a fume hood.

Thin Layer Chromatography

UV lamp, capillary tube, ml beaker Materials: TLC is a quick and inexpensive technique that can be used to 1 determine the number of compounds in a mixture, 2 identify the compounds, 3 monitor the progress of a reaction, 4 determine the effectiveness of a purification, 5 determine the appropriate conditions for column chromatography separation, and 6 analyze the fractions obtained from column chromatography.

Seven different solvent mixtures were compared in order to evaluate which mixture was most effective as an elution solvent in separating four unknown compounds. If an eluent traveling through a silica gel is less polar, the less polar component will travel down the gel quickly for collection, while the polar component stays stationary in the gel.

A developing chamber was prepared by using a ml beaker, a half-piece of filter paper inside and aluminium foil to cover. The process of washing a compound through a column using a solvent is known as elution. In the case of an optimal separation, different peaks or patterns on the chromatogram correspond to different components of the separated mixture.

The plates were observed the first fraction being on the left, the second fraction being in the middle, and the third fraction being on the right as follows: Problems and Solutions Figure 3: Watch movie how to pull capillaries here here Spotting the plate The thin end of the spotter is placed in the dilute solution; the solution will rise up in the capillary capillary forces.

Thin Layer Chromatography and Column Chromatography Experiment

Hold the plate face up 10 to 20 cm above the heat gun until the bulk water evaporates.Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures.

Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or layer of adsorbent is known as.

The mobile phase moves through the chromatography column (the stationary phase) where the sample interacts with the stationary phase and is separated.

Preparative chromatography is used to purify sufficient quantities of a substance for further use, Thin layer chromatography (TLC).

Thin Layer and Column Chromatography

Thin-layer chromatography is a type of liquid chromatography in which the stationary phase is in the form of a thin layer on a flat surface rather than packed into a tube (column). It is a member of a family of techniques that include some types of electrophoresis and paper chromatography more generally referred to as planar chromatography.

Thin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. It may be performed on the. In thin layer chromatography, the stationary phase is a thin layer of silica gel or alumina on a glass, metal or plastic plate.

Column chromatography works on a much larger scale by packing the same materials into a vertical glass column.

Thin-layer chromatography

3 Column Chromatography In column chromatography, the stationary phase is packed into a vertical tube. Apply the sample to the top of the column and allow the mobile phase to percolate down through the stationary phase.

Thin layer chromatography and column chromatography
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